MICHAELIS-MENTEN AND LINEWEAVER BURKE PLOT
Students should plot a Michaelis-Menten and Lineweaver Burke plot based on the following questions: For Michaelis-Menten plot: This data is obtained from the conversion of cellulose to cellobiose by endoglucanase enzyme: Concentration of Substrate [S] 0.5 0.75 2 4 6 8 10 Velocity (V) 0.075 0.09 0.152 0.196 0.21 0.214 0.23 a) Using the above data, plot a graph on a properly labelled and titled Michaelis-Menten plot. b) From the graph, calculate the Vmax and Km value of the enzyme. For Lineweaver Burke plot: This data is obtained from the conversion of amylopectin to β-limit dextrin by isoamylase enzyme: [S] (mM) 2.5 1 0.714 0.526 0.250 velocity (mM.s -1 ) 0.588 0.500 0.417 0.370 0.256 a) Using the above data, recalculate and plot a graph on a properly lablled & titled Lineweaver Burk plot. b) Using the graph, calculate the Vmax and Km value of the enzyme. c) On the grapgh above, draw and lab