BOSSKU BIOCHEMISTRY

Students should plot a Michaelis-Menten and Lineweaver Burke plot based on the following questions: For Michaelis-Menten plot: This data is obtained from the conversion of cellulose to cellobiose by endoglucanase enzyme: Concentration of Substrate [S] 0.5 0.75 2 4 6 8 10 Velocity (V) 0.075 0.09 0.152 0.196 0.21 0.214 0.23 a) Using the above data, plot a graph on a properly labelled and titled Michaelis-Menten plot. b) From the graph, calculate the Vmax and Km value of the enzyme. For Lineweaver Burke plot: This data is obtained from the conversion of amylopectin to β-limit dextrin by isoamylase enzyme: [S] (mM) 2.5 1 0.714 0.526 0.250 velocity (mM.s -1 ) 0.588 0.500 0.417 0.370 0.256 a) Using the above data, recalculate and plot a graph on a properly lablled & titled Lineweaver Burk plot. b) Using the graph, calculate the Vmax and Km value of the enzyme. c) On the grapgh above, draw and...

 Protein Structure Several level of protein structure  1.   Primary structure – sequence of amino acid.  2.   Secondary structure – local structure.  3.   Tertiary structure – overall 3D shape of protein.  4.   Quarternary structure – subunit organization Tertiary structure - 2 examples of tertiary structures are fibrous and globular protein. Fibrous protein vs Globular protein Fibrous Globular Called as scleroproteins. Elongated strand-like structures and are usually in the form of rods or wires. Called as spheroproteins owing to their shape. Spherical in shape and has the property of forming colloids with water. It insoluble in water, weak acids and weak bases but soluble in strong acids and alkalis It soluble in water, acids and bases The peptide chains are bound together by strong intermolecular hydrogen bonds in fibrous proteins. The peptide chains are bound together by weak intermole...

The Henderson - Hasselbalch Relationship   The Henderson - Haseelbalch equation is used to determine the final pH of a weak acid solution once the dissociation reaction reaches equilibrium.            Calculate the pH of a mixture of 0.1 M acetic acid and 0.2 M sodium acetate. (The pKa of acetic is 4.76).

NAZRUL SYAZWAN BIN MOHD NAZERI 72461 STB1083 BIOCHEMISTRY AQMAL SYABIL BIN ASMADY (72125)   MUHAMMAD HANIF FIKRI BIN MOHD KHIRUDIN (72417)